Targeted Gene Editing in Porcine Spermatogonia

Genome Editing With Targeted Deaminases

Luhan Yang, Adrian W. Briggs*, Wei Leong Chew*, Prashant Mali, Marc Guell, John Aach, Daniel Bryan Goodman, David Cox, Yinan Kan, Emal Lesha Venkataramanan Soundararajan, Feng Zhang, George Church 1 Department of Genetics, Harvard Medical School, Boston, MA 2 Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, MA 3 Harvard-MIT Division of Health Science and Technology...

The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation.

The CRISPR/Cas9 system has been demonstrated to efficiently induce targeted gene editing in a variety of organisms including plants. Recent work showed that CRISPR/Cas9-induced gene mutations in Arabidopsis were mostly somatic mutations in the early generation, although some mutations could be stably inherited in later generations. However, it remains unclear whether this system will work simil...

Targeted gene editing by transfection of in vitro reconstituted Streptococcus thermophilus Cas9 nuclease complex

Cas9 protein of the Type II CRISPRCas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated) bacterial adaptive immune system emerged recently as a promising tool for genome editing in human and other eukaryotic cells. Cas9 binds a dual crRNA (CRISPR RNA)-tracrRNA (transactivating RNA) molecule, or an artificial single-guide RNA (sgRNA) into a functional complex that acts...

Targeted gene knock-in by homology-directed genome editing using Cas9 ribonucleoprotein and AAV donor delivery

Realizing the full potential of genome editing requires the development of efficient and broadly applicable methods for delivering programmable nucleases and donor templates for homology-directed repair (HDR). The RNA-guided Cas9 endonuclease can be introduced into cells as a purified protein in complex with a single guide RNA (sgRNA). Such ribonucleoproteins (RNPs) can facilitate the high-fide...

Targeted gene editing in the sea urchin embryo using zinc-finger nucleases